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Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications. Kod Xtreme Hot Start Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more 71842. ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR. Sigma-Aldrich.
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Hot start PCR follows the same principles as the conventional PCR - in that it uses DNA polymerase to synthesise DNA from a single stranded template, however, it utilises additional heating and separation methods, such as inactivating or inhibiting the binding of Taq polymerase and late addition of Taq polymerase, to increase product yield as well as provide a higher specificity and sensitivity. This protocol uses MilliporeSigma's KOD Xtreme™ Hot Start DNA Polymerase, which allows for high fidelity PCR using a simple three-step thermocycle.
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KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications.
: Denna produkt är en blandning. Produkten är inte något farligt
20 okt. 2010 — Detta har företaget Novagen tagit fasta på då man utvecklat polymeraset och PCR-systemet Kod Xtreme Hot Start DNA Polymerase Kit.
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Material Safety Data Sheet or SDS for KOD Xtreme™ Hot Start DNA Polymerase 71975 from MilliporeSigma for download or viewing in the browser. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively.
KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. KOD Xtreme™ Hot Start DNA Polymerase Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates. - Find MSDS or SDS, a COA, data sheets and more information. Se hela listan på sigmaaldrich.com
KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.
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KOD Xtreme™ Hot Start DNA Polymerase VWR
The 3-D structure of DNA polymerase was characterized in 2003 (Fig. 5) (3).
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The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The KOD Xtreme™ Hot Start DNA Polymerase is a ready-to-use reaction or routine PCR amplification, and optimized for difficult targets Efficiently amplifies up to 90% GC-content DNA templates, genomic targets up to 24 kbp and phage/Lambda targets up to 40 kbp. High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. KOD Hot Start DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.